Tested applications
ICC/IF, WB, Flow Cyt, IHC-P
Cross reactivity
Reacts with
Human
Overview
Product name
Anti-Flt3 ligand antibody [EP1140Y]
See all Flt3 ligand products （6） ...
Description
Rabbit monoclonal [EP1140Y] to Flt3 ligand
Specificity
The antibody has been tested against recombinant protein in western blotting, we do not have data regarding recognition of endogenous protein. We suggest K562 and HL-60 cells could be expressing Flt3 ligand. The antibody was raised against the extracellular domain of the protein, however please note permeabilisation in FACS greatly improves the signal.
Tested applications
ICC/IF, WB, Flow Cyt, IHC-Pmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues within human Flt3 ligand.
Positive control
IHC: Human tonsil tissue. WB: recombinant protein
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Research areas
Immunology >> Innate Immunity >> Cytokines >> Other
Cardiovascular >> Angiogenesis >> Cytokines >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
ICC/IF
-
1 Image
WB
-
Flow Cyt
-
1 Image
IHC-P
Application notes
Flow Cyt: 1/10.
ICC/IF: 1/100 - 1/250.
IHC-P: 1/50 - 1/100. Antigen retrieval recommended.
WB: 1/100000. Detects a band of approximay 22 kDa （predicted molecular weight: 26 kDa）.
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionP49771The UniProt Consortium
The Universal Protein Resource （UniProt） in 2010
Nucleic Acids Res. 38:D142-D148 （2010）.
Information by UniProt
Alternative names
- Flt 3 ligand antibody
- Flt 3L antibody
- Flt3 L antibody
- FLT3 LG antibody
- Flt3 ligand antibody
- Flt3L antibody
- FLT3L_HUMAN antibody
- FLT3LG antibody
- Fms related tyrosine kinase 3 ligand antibody
- Fms-related tyrosine kinase 3 ligand antibody
- SL cytokine antibody
see all
Database links
- Entrez Gene: 2323 Human
- Omim: 600007 Human
- SwissProt: P49771 Human
- Unigene: 428 Human
Anti-Flt3 ligand antibody [EP1140Y] images:
Western blot - Flt3 ligand antibody [EP1140Y] （ab52648）
![Western blot - Flt3 ligand antibody [EP1140Y] （ab52648）](https://img73.foodjx.com/bb33f2c9b6dc3b85eb515dd667773c4ad640e4a410fe60f1915db1e01f6f40214854aa05c128733e.jpg)
[EP1140Y] （ab52648） at 1/100000 dilution + Recombinant Flr3 ligand at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 26 kDa
Observed band size : 22 kDa （why is the actual band size different from the predicted?）
Immunohistochemistry （Paraffin-embedded sections） - Flt3 ligand antibody [EP1140Y] （ab52648）
![Immunohistochemistry （Paraffin-embedded sections） - Flt3 ligand antibody [EP1140Y] （ab52648）](https://img73.foodjx.com/bb33f2c9b6dc3b85eb515dd667773c4ad842887e73e250670ba49367744d1e49722791bc7de304f6.jpg)
Immunohistochemical staining of paraffin-embedded human tonsils using ab52648 at a 1/50 dilution.
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